Human Anti-Vd2 Antibody Gives Good Results

April 22, 2021

Columbia Centre for Translational Immunology
Columbia University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Vd2 Pacific Blue mouse Anti-Human

Catalog Number:

331414

Clone Number:

B6

I was able to determine Gammadelta T cell subsets (Vd1 and Vd2) with this pacific blue Vd2 antibody upon initial staining. No optimization was required and the antibody displayed great separation and perfect resolution at a concentration of 1:100

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human blood, spleen and lung samples

Primary Incubation

1:100, 30 minutes 4°C

Blocking Agent

FC Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

I managed to detect an expanded population of highly cytotoxic Vd2+ Gammadelta T cells in the lungs of pediatric donors in comparison to the spleen and blood

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Perfect resolution. Uncommonly used color in recent times so fitted into a complex spectral flow cytometry panel with fluorophores such as BV421 and BV480

The Bad

Nothing

The Bottom Line

This antibody worked perfectly with minimal optimization on what is typically considered a dim fluorophore

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