MdM2 Antibody Works Well

Biochemistry
Heidelberg University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell signaling technology (CST)

Product Name:

MdM2 antibody

Catalog Number:

#86934

Clone Number:

D1V2Z

A western blot was used to check the expression of MDM2 after a specific treatment in Hela cells

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela cells, cell lysate

Primary Incubation

1:1000 overnight

Blocking Agent

Milk 5% in PBS 0,05% tween

Secondary Incubation

Rabbit 1:3000 milk 1% in PBS 0,05% tween 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

The bands are visible but the background of the antibody is a big too high for the price.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Dirty background

The Bottom Line

I would search for something better

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