Miltenyi Biotec
IgM Antibody, anti-mouse, REAfinity
130-116-215
REA979
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining IgM on the surface of B cells.
Flow Cytometry
Mouse WT spleen
Surface staining with anti-IgM (1/25), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We could stain IgM very well.
Reliable and good signal
I would buy it again.