Reliable Antibody to Identify SARS-CoV-2 Using ELISA

Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sino Biological

Product Name:

SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified

Catalog Number:

40592-T62

Clone Number:

N/A

A receptor-binding domain (RBD) is a key part of SARS-CoV-2 located on its ‘spike’ domain that allows it to dock to ACE2 to gain entry into cells and lead to infection. We use anti-RBD antibodies from sino biological to detect spike protein.

Experimental Design and Results Summary

Applications

ELISA

Sample

Patient samples, recombinant RBD proteins

Primary Incubation

Dilution: 1:6000 Time: 60 min. Temp: Room Temperature

Blocking Agent

1% BSA in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Anti-rabbit IgG, HRP-linked Antibody. Detection at 450 nm after stopping the reaction.

Results Summary

This antibody is a good choice against RBD of spike protein. Antibody is very specific and works very well in the suggested dilution.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Antibody is very specific. Less background noise. Results are reproducible.

The Bad

N/A

The Bottom Line

This antibody is a very good choice to detect the spike RBD domain.

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