Biolegend
Alexa Fluor 647 anti-mouse CD19 Antibody
115522
6D5
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) in health and disease. Here, we wanted to stain B cells (CD19+).
Flow Cytometry
Spleen of Blimp-1eGFP naive
surface staining with CD19 A677 (1/200), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We could clearly stain B cells (CD19+).
It work well
Good option to stain mouse CD19