Ok Option to Stain Red Blood Cells (RBCs)

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

Miltenyi Biotec

Product Name:

Ter-119 Antibody, anti-mouse, VioBlue, REAfinity

Catalog Number:

130-112-914

Clone Number:

REA847

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) in health and disease, although in this particular experiment, we are looking for red blood cells (Ter119+).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse spleen before and after red blood cell (RBC) lysis

Primary Incubation

Ter119 VioBlue (1/50), 4°C, 20min

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

As it can be observed in the figure, we found a significative reduction in RBC (Ter119+) after RBC lysis.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It works

The Bad

It is not very bright

The Bottom Line

Ok option

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