Good Anti-Mouse CD4

January 25, 2021

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

Miltenyi Biotec

Product Name:

anti-mouse CD4 Antibody VioGreen

Catalog Number:

130-118-693

Clone Number:

REA604

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) in health and disease, although in this particular experiment, we are looking for CD4+ T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57Bl/6 spleen

Primary Incubation

anti-CD4 viogreen (1/200), 4C, 20 min in the dark

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could identify CD4 T cells in the spleen.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It works

The Bad

Could be brighter

The Bottom Line

Good option!

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