CXCL8 Expressed in Fibroblasts

March 18, 2021

BlueskyReddit
UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Bioscience

Product Name:

PE-CF594 Mouse Anti-Human IL-8 PE-CF594 Mouse Anti-Human IL-8

Catalog Number:

563531

Clone Number:

N/A

The goal was to detect the expression of CXCL8 in skin fibroblasts. The antibody staining indicated that all fibroblast expressed CXCL8.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

skin fibroblasts

Primary Incubation

1/20, 4C for 15min

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

The antibody detected all fibroblasts expressing CXCL1. The cells needed to be fixed and permeabilized before using the antibody.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to sue

The Bad

None

The Bottom Line

It works well.

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