Good Antibody for MUC2 IF Staining

MIT
MIT
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

MUC2 Recombinant Rabbit Monoclonal Antibody (JA10-92)

Catalog Number:

MA5-32654

Clone Number:

N/A

We aimed to stain MUC2, which is the most abundant mucin secreted by goblet cells that plays a pivotal role in organizing the intestinal mucus layers and barrier integrity at the epithelial apical surface.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Caco2 cells

Primary Incubation

1:100

Blocking Agent

Donkey serum

Secondary Incubation

1:500 AF488

Tertiary Incubation

N/A

Detection

Confocal microscopy

Results Summary

We were able to observe intestinal mucin protein in the Caco2 cells cultured for 7 days in microfluidic devices.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

strong signal

The Bad

Hard to optimize

The Bottom Line

This antibody is recommended for in vitro assays but needs to be checked for the specific cell line you are working with.

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