Staining Peripheral Blood with anti-CD3 Alexa488

January 11, 2021

BlueskyReddit
Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

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Company:

Biolegend

Product Name:

anti-CD3 Alexa488

Catalog Number:

300320

Clone Number:

HIT3a

This antibody was used to stain for T cells in human peripheral blood. It was chosen because it is a commonly used clone for CD3, Alexa488 is bright and works well with other fluorophores in the staining panel, and Biolegend has a good reputation for making quality flow cytometry antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood

Primary Incubation

5 ul per test in 100uL staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 0.2% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD3 staining was excellent. Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well. Reproducible results.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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