Conjugated CD49f PerCP-eFluor 710 Antibody is Great for Flow Cytometry

January 09, 2021

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Anti-human/mouse CD49f PerCP -eFluor 710

Catalog Number:

46-0495-82

Clone Number:

N/A

Human cervical cancer cells were stained with CD49f antibody and sorted for CD49f- positive and -negative cells. CD49f antibody is a cancer stem cell marker so positive cells were evaluated for tumorsphere growth.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human cervical cancer cells

Primary Incubation

1:200, 1 hour, on ice

Blocking Agent

ALDEFLUOR Binding Buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Cervical cancer cells were effectively stained and showed co-expression with ALDEFLUOR, indicating presence of potential cancer stem cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very easy to use.

The Bad

Optimization required for evaluation of expression.

The Bottom Line

CD49f can be co-stained with other cancer stem cell markers, including ALDEFLUOR. ALDEFLUOR must be performed at 37 degrees while CD49f must be done subsequently on ice to prevent efflux of ALDEFLUOR. Make sure to have cells stained only with CD49f as control for proper Flow Cytometry gating.

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