Biolegend
APC anti-mouse CD319 Antibody
152003
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) in health and disease. In this particular experiement, we aimed at staining PCs with anti-CD319.
Flow Cytometry
mouse spleen - Blimp-1eGFP KI
surface staining with CD138 BV421, anti-CD319 APC (1/800), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We found that PCs (Blimp-1+CD138+) express CD319.
This antibody works really well.
Very good option to stain mouse CD319