Anti-CD3 PE Works Really Well

January 06, 2021

BlueskyReddit
Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

anti-CD3 PE

Catalog Number:

550353

Clone Number:

1F4

This antibody was used as a marker for T cells in splenocytes isolated from Sprague-Dawley rats. It was chosen because it is a commonly used clone for CD3, PE is bright and works well with other fluorophores in the staining panel, and BD has a good reputation for making quality flow cytometry antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat splenocytes

Primary Incubation

1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD3 staining was excellent. Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well. Reproducible results.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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