Cell Strainer for Single Cell Suspension

Centre for Cellular & Molecular Biology
Project JRF
Postdoctoral Researcher

Overall

Performance

Ease-of-Optimization

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Company:

Corning

Product Name:

Falcon 70uM cell strainer

Catalog Number:

352350

A characteristic phenotype of normal or a cancer stem cell is their ability of divide or proliferate several times. To study this property of a stem cell in-vitro, we need to isolate the niche and plate them as single cells in the culture dish. Plated single cells divide several times to form a sphere in the culture. Measuring diameter of the sphere at the end of the assay reveals the proliferative efficiency of the stem cell. Hence, preparing single cell suspension is a very important initial step which can be achieved using the cell strainer. This Falcon cell strainer prevents clumped cells and permeabilize single cells only. This cell strainer is also useful in making suspension for flow cytometric assays.

Experimental Design and Results Summary

Application

Stem cell studies and Flow cytometric assays

Tips

After opening the pack, try to pick the strainer from the sleeve provided. Touching the mesh or the bottom of the stainer can lead to contaminations

DOI or PMID #

N/A

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Summary

The Good

Prevents cell clumps efficiently

The Bad

Very rarely the suspension doesn't pass through if there is airlock between the stainer and the falcon tube.

The Bottom Line

Corning - Falcon cell strainer is an highly recommended material for making suspension with isolated single cell.

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