Measuring Rab3d Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Abnova

Product Name:

RAB3D polyclonal antibody (A01)

Catalog Number:

H00009545-A01

Clone Number:

Rab proteins are called as GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. It is located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver. Here we checkdd the time course effect of chemoattractant on Rab 3d expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of Rab3d expression by chemoattractant.

DOI or PMID #

Additional Notes

Related Categories

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Summary

The Good

Highly specific and strong signal

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

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