Great Results

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Sigma Aldrich

Product Name:

Monoclonal Anti-γ-Tubulin antibody

Catalog Number:

T5326

Clone Number:

N/A

Info: Supplier Page 

Browse Similar Products: y tubulin Western Blot Antibodies

γ-Tubulin is a member of the tubulin family. It is localized to the microtubule organizing centres.γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. Here we checked the time course effect of γ-Tubulin by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We did not found any time dependent increase in γ-Tubulin expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody is very specific and the signal is very strong

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion