Measuring ACE2 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling

Product Name:

ACE2 (OTI1G4)

Catalog Number:

74512

Clone Number:

N/A

Info: Supplier Page 

Browse Similar Products: ACE2 Western Blot Antibodies

Angiotensin-converting enzyme 2 (ACE2) is an enzyme present in the cell membranes of cells located in the lungs, arteries, heart, kidney, and intestines. ACE2 lowers blood pressure by catalyzing the hydrolysis of angiotensin II (a vasoconstrictor peptide) into angiotensin (a vasodilator). ACE2 counters the activity of the related angiotensin-converting enzyme (ACE) by reducing the amount of angiotensin-II and increasing Ang(1-7), making it a promising drug target for treating cardiovascular diseases. ACE2 also serves as the entry point into cells for some coronaviruses, including HCoV-NL63, SARS-CoV, and SARS-CoV-2. Here we checked the time course effect of ACE2 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 dilution in 5% Milk prepared in 1x TBST

Blocking Agent

5% Milk prepared in 1x TBST

Secondary Incubation

1:4000 dilution in 5% Milk prepared in 1x TBST

Tertiary Incubation

NONE

Detection

ECL

Results Summary

We found time dependent increase of ACE2 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and highly specific

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

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