Good Antibody for IP and ChIP

Gurdon Institute
University of Cambridge
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Pol-II Ser2

Catalog Number:

ab26721

Clone Number:

N/A

I have used this Antibody in studying the regulation of RNA-Pol-II in the Xenopus oocytes. The results of ChIP were very satisfactory. Although recommend using the phos-inhibitors

Experimental Design and Results Summary

Applications

ChIP

Sample

Xenopus oocytes

Primary Incubation

1ug per IP

Blocking Agent

BSA

Secondary Incubation

24 hrs

Tertiary Incubation

Not available

Detection

Protein A/G

Results Summary

Elongation of Pol-II can be seen on an injected plasmid in the oocyte as soon as in 30 minutes. The green line shows the enrichment of Pol-II ser2 II at the injected plasmid.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to optimise and use

The Bad

Needs some extra care with experiments.

The Bottom Line

I recommend this product for Pol-II elongation studies

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