CD105 Works Well

D-BSSE
ETH Zurich
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse CD105 Antibody

Catalog Number:

120414

Clone Number:

MJ7/18

This antibody is used to stain CD105 antigen on mouse hematopoietic cell types and red blood cell types using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse - Bone marrow

Primary Incubation

2 microgram per mouse at 4' C for 60 min

Blocking Agent

PBS + 2 % FCS

Secondary Incubation

Not required

Tertiary Incubation

Not required

Detection

Flow cytometry

Results Summary

This antibody is used to stain CD105 antigen on mouse hematopoietic cell types and red blood cell types using flow cytometry. It enables the analysis, isolation and purification of these cell types from mouse bone marrow.The antibody is easy to use, has high signal to noise ratio and can also be used for live cell imaging. Also, antibody does not spill over into other channels except APCCY7 channel.Highly recommend this antibody, particularly when there are limited possibilities of CD105 antibodies for flow cytometry.

DOI or PMID #

https://doi.org/10.1016/j.stemcr.2020.06.008

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nicely stains hematopoietic and red blood cell types with high signal to noise ratio

The Bad

Weak signal during live imaging

The Bottom Line

Recommend this antibody overall owing to low price and high signal to noise ratio

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