Using IgA to Detect Plasma Cells

December 20, 2020

BBM III e Inmunologia
Universtity of Granada
Post-doc

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Quality of Results

Ease-of-Optimization

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Company:

Thermofisher

Product Name:

IgA Monoclonal Antibody (mA-6E1), PE

Catalog Number:

12-4204-82

Clone Number:

mA-6E1

We are using the IgA antibody to characterize the immune cells populations in the mouse intestine during chronic inflammation. This antibody is perfect to clearly separate IgA+ cells from other immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lamina propia cells from normal mice

Primary Incubation

Dilution: 1:500 Time: 20 min. Temp: 4C

Blocking Agent

1% BSA supplemented with 4% of mouse serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Lamina propia cells were analysed by flow citometry using a FACSCanto II (BD).

Results Summary

This antibody is a good choice in combination with the B220 to detect lamina propria plasma cells during chronic imflammation

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It is a bright marker that allows you to detect clearly the B cell population

The Bad

Nothing remarkable

The Bottom Line

This antibody is highly recommended

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