Using CD3 to Detect T Cells

BBM III e Inmunologia
Universtity of Granada
Post-doc

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Company:

Biolegend

Product Name:

FITC anti-mouse TCR β chain Recombinant Antibody

Catalog Number:

159705

Clone Number:

QA18A18

We are using the TCRb antibody to characterize the immune cells populations in the mouse intestine during chronic inflammation. This antibody is perfect to clearly separate conventional T cells from other immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lamina propia cells from normal mice

Primary Incubation

Dilution: 1:500 Time: 20 min. Temp: 4C

Blocking Agent

1% BSA supplemented with 4% of mouse serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Lamina propia cells were analysed by flow citometry using a FACSCanto II (BD).

Results Summary

This antibody is a good choice in combination with the anti-CD3 to detect lamina propria T cells during chronic imflammation

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It didn't interfered with the other markers from my FACS panel.

The Bad

It is not as bright as I would like.

The Bottom Line

This antibody is a good choice to detect T cells.

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