Using B220 to Detect B Cells

December 21, 2020

BlueskyReddit
BBM III e Inmunologia
Universtity of Granada
Post-doc

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Company:

Biolegend

Product Name:

PE/Dazzle™ 594 anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103258

Clone Number:

RA3-6B2

We are using the B220 antibody to characterize the immune cells populations in the mouse intestine during chronic inflammation. This antibody is perfect to clearly separate B cells from other immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lamina propia cells from normal mice

Primary Incubation

Dilution: 1:500 Time: 20 min. Temp: 4C

Blocking Agent

1% BSA supplemented with 4% of mouse serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Lamina propia cells were analysed by flow citometry using a FACSCanto II (BD).

Results Summary

This antibody is a good choice to detect B cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It is a bright marker

The Bad

Nothing remarkable

The Bottom Line

This antibody is a good choice in combination with the anti-CD45 to detect lamina propria B cells.

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