Using CD45 to Detect Immune Cells

December 22, 2020

BlueskyReddit
BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

APC/Cyanine7 anti-mouse CD45 Antibody

Catalog Number:

103116

Clone Number:

30-F11

We are using the APC/Cyanine7 anti-mouse CD45 antibody to characterize the immune cells populations in the mouse intestine during chronic inflammation. This antibody allows to separate immune cells from the intestinal stromal cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lamina propia cells from normal mice

Primary Incubation

Dilution: 1:500 Time: 20 min. Temp: 4C

Blocking Agent

1% BSA supplemented with 4% of mouse serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Lamina propia cells were analysed by flow citometry using a FACSCanto II (BD).

Results Summary

Identification of immune cells from mouse ileum

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It is a bright marker

The Bad

Nothing remarkable

The Bottom Line

This antibody is a good choice to detect immune cells.

Share your experience with other scientists. Write a Review! »

Join the discussion