Using CD11b to Detect Myeloid Cells

January 11, 2021

BlueskyReddit
BBM III e Inmunologia
Universtity of Granada
Post-doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

PE anti-mouse/human CD11b Antibody

Catalog Number:

101208

Clone Number:

M1/70

We are using the CD11b-PE antibody to characterize the immune cells populations in the mouse intestine during chronic inflammation. This antibody is perfect to identify myeloid cells in the intestine.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lamina propia cells from normal mice

Primary Incubation

Dilution: 1:500 Time: 20 min. Temp: 4C

Blocking Agent

1% BSA supplemented with 4% of mouse serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Lamina propia cells were analysed by flow citometry using a FACSCanto II (BD).

Results Summary

This antibody is a good choice to detect lamina propria myeloid cells during chronic imflammation

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

It is a bright marker that allows you to detect clearly the myeloid populations

The Bad

Nothing remarkable

The Bottom Line

This antibody is a good choice in combination with other antibodies, such as Ly6G or CD64, to characterize lamina propria myeloid cells during chronic imflammation

Share your experience with other scientists. Write a Review! »

Join the discussion