Using Ly6G to Detect Neutrophils

January 29, 2021

BlueskyReddit
BBM III e Inmunologia
Universtity of Granada
Post-doc

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Company:

Thermofisher

Product Name:

Ly-6G Monoclonal Antibody (1A8-Ly6g), Biotin

Catalog Number:

13-9668-82

Clone Number:

1A8-Ly6g

We are using the Ly6G antibody to characterize the immune cells populations in the mouse intestine during chronic inflammation. This antibody is perfect to clearly separate Neutrophils from other immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lamina propia cells from normal mice

Primary Incubation

Dilution: 1:500 Time: 20 min. Temp: 4C

Blocking Agent

1% BSA supplemented with 4% of mouse serum

Secondary Incubation

Strept. PE/Cy5. Dilution: 1:500 Time: 20 min. Temp: 4C

Tertiary Incubation

None

Detection

Lamina propia cells were analysed by flow citometry using a FACSCanto II (BD).

Results Summary

Lamina propia neutrophils were identified as CD45+ CD11b+ and Ly6G+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It is a bright marker that allows you to detect clearly the Neutrophil population

The Bad

Nothing remarkable

The Bottom Line

This antibody is a good choice in combination with the anti-Cd11b to detect lamina propria neutrophils during chronic inflammation

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