Detect Tbet Expression in Human T Cells

Microbiology and Immunology
Columbia University Irving Medical Center
Postdoctoral Researcher

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Company:

Biolegend

Product Name:

Brilliant Violet 785 anti-T-bet Antibody 4b10

Catalog Number:

644835

Clone Number:

4B10

Our lab studies development and function of human immune cells. We utilize flow cytometry to identify and study cell population of interest in different tissues of the human body. We used this antibody to examine Tbet expression on human spleen T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Spleen Cells

Primary Incubation

1:50, 60min on RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora

Results Summary

Antibody works well to show good intracellular expression of Tbet in human spleen T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody in good color

The Bad

None

The Bottom Line

Bright antibody that works well and highly recommended.

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