Reliable Viability Dye

Columbia
Microbiology and Immunology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD

Product Name:

Fixable Viability Stain 700

Catalog Number:

564997

Our lab studies T cell biology in human and mice. Flow cytometry is an essential tool we use to examine various immune cells in tissues of human and mice. Therefore it is crucial for us to label dead cells that can be gated out for our analysis.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Mouse lung cells

Tips

Used at 1:1000, and stain in PBS for best result

Results Summary

We can clearly identify dead cells (positive) from our flow analysis and gate them out for further analysis.

DOI or PMID #

N/A

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Summary

The Good

Labels dead cells effectively and can be fixed for further intracellular staining

The Bad

Staining of this dye requires no serum present for best optimal signal.

The Bottom Line

This is a essential dye used for almost all flow experiments.

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