Human CD3 Antibody Works Well

December 22, 2020

Microbiology and Immunology
Columbia
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

CD3

Catalog Number:

317324

Clone Number:

OKT3

Our lab studies development and function of human immune cells. We utilize flow cytometry to identify and study cell population of interest in different tissues of the human body.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human blood

Primary Incubation

1:100, 30min on ice

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII

Results Summary

Antibody enabled identification of T cells in human blood and lymph nodes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well with good signal separation.

The Bad

Slight background issue

The Bottom Line

This is a go-to antibody for most of my stain as the fluorophore and signal is very reliable.

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