Anti-Rab5 Antibody That Works Great for IF

Public health
Rice University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Rab5 (C8B1) Rabbit mAb

Catalog Number:

#3547

Clone Number:

Rab5 (C8B1)

We used his antibody to detect GFP-fused proteins in MCF-10A cell line for immunofluorescence staining. It worked very well.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human cell lines

Primary Incubation

1:250 4 degree over night

Blocking Agent

5% BSA

Secondary Incubation

1:1000

Tertiary Incubation

None

Detection

Confocal microscopy.

Results Summary

The results are strong and clear. Only minimal background staining could be detected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific for target protein.

The Bad

None

The Bottom Line

I highly recommend this for immunofluorescence staining. It works very well.

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