RNeasy Plus Mini Kit Works Well

New York University
Biology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

QIAGEN

Product Name:

RNeasy plus mini kit

Catalog Number:

74134

The RNeasy plus mini kit can be used to extract total RNA from dried blood spots on filter paper. This QIAGEN kit includes gDNA removal columns for the elimination of DNA from all samples as well as the standard QIAGEN spin columns used for the isolation and washing of the RNA sample(s).

Experimental Design and Results Summary

Application

RNA extraction and isolation

Starting Material

Dried blood spots (whole blood) on filter paper

Tips

Standard precautions and care should be taken when working with RNA and human samples.

Results Summary

The quality and quantity of RNA isolated from each sample can be determined by Nano drop and/or Qubit.

DOI or PMID #

N/A

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Summary

The Good

Easy and intuitive protocol; High and clean yield; Time efficient; Standardized method.

The Bad

Price per sample / expensive.

The Bottom Line

The QIAGEN RNeasy kit is an efficient and effective method for the isolation of clean and high yield RNA from dried blood spot samples archived on filter paper.

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