CD41-BV605 Works Well

February 18, 2021

BlueskyReddit
D-BSSE
ETH Zurich
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV605 Rat Anti-Mouse CD41

Catalog Number:

747728

Clone Number:

MWReg30

Info: Supplier Page 
Browse Similar Products: CD41 Flow Cytometry Antibodies

This antibody stains CD41 antigen of mouse hematopoietic stem and progenitor cells and mature cells. Particularly, it stains megakaryocytes and their progenitors with nice efficiency and allows their analysis, isolation and purification.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse - Bone marrow

Primary Incubation

1 microgram per mouse at 4' C for 60 min

Blocking Agent

PBS + 2 % FCS

Secondary Incubation

Not required

Tertiary Incubation

Not required

Detection

Flow cytometry

Results Summary

This antibody nicely stains CD41 antigen of mouse hematopoietic stem and progenitor cells, particularly megakaryocytes, and allows their analysis, isolation and purification using flow cytometry.

DOI or PMID #

Not applicable

Additional Notes

Not applicable

Image Gallery

Summary

The Good

Nice signal and low concentration required

The Bad

Spills a lot into other UV channels e.g. BV650, BV711, and particularly PE and mCherry channels

The Bottom Line

Recommend this antibody when not many antibodies are used to get nice CD41 signal and to avoid spill over into other channels.

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