Sca1-Pacific Blue

D-BSSE
ETH Zurich
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Sca1-Pacific blue

Catalog Number:

108120

Clone Number:

D7

This antibody is optimal to stain stem cell antigen (Sca-1) on mouse hematopoietic and progenitor cells and allows the analysis, isolation and purification of these cell types using FACS sorter.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse - Bone marrow

Primary Incubation

2-3 microgram per mouse at 4' C for 60 min

Blocking Agent

PBS + 2 % FCS

Secondary Incubation

Not required

Tertiary Incubation

Not required

Detection

Flow cytometry

Results Summary

The antibody nicely separates blood stem cells from progenitors of red blood cells, monocytes, neutrophils, megakaryocytes etc using Flow cytometry. Hence, it allows analysis, isolation and purification of mouse blood stem cells.

DOI or PMID #

Not applicable

Additional Notes

Not applicable

Related Categories

Image Gallery

Summary

The Good

Nice signal with minimum spill over into other channels.

The Bad

Weak signal. Need high concentration of this antibody.

The Bottom Line

Highly recommend this antibody to stain mouse blood stem and progenitor cells.

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