Good Antibody to Detect CD4+ T Cells Through Flow

February 17, 2021

BlueskyReddit
Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Rat Anti-Mouse CD4-Alexa Fluor 647

Catalog Number:

557681

Clone Number:

RM4-5

This antibody is used to detect CD4+ T cells using flow cytometry. These cells are helper cells of the adaptive immune system.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor lymphocytes

Primary Incubation

1:500

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

We can easily identify CD4+ T cells in the tumor.

DOI or PMID #

NA

Additional Notes

Titrate antibody based on tissue samples

Related Categories

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Summary

The Good

Robustly identifies CD4 T cells in the tumor.

The Bad

None

The Bottom Line

Great antibody especially if used with spectral flow cytometers since can use APC with AF647.

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