Reliable Antibody to Identify CD19+ B Cells using Flow Cyometry

January 27, 2021

BlueskyReddit
Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

anti-mouse CD19 Antibody-PE/Cy5

Catalog Number:

115510

Clone Number:

B4

B cells are an important cell type in the adaptive immune system. CD19 is a marker typically used to identify B cell subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine splenocytes

Primary Incubation

1:500

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

We can easily distinguish CD19+ B cells from other T cells.

DOI or PMID #

N/A

Additional Notes

Titrate antibody based on tissue samples

Related Categories

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Summary

The Good

Robustly identifies CD19+ B cells in the tumor.

The Bad

None

The Bottom Line

Great antibody. Recommend if using spectral flow.

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