Great Antibody to Identify CD137 Expression using Flow Cytometry

January 19, 2021

BlueskyReddit
Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher eBioscience

Product Name:

CD137 (4-1BB) Monoclonal Antibody-PerCP-eFluor 710

Catalog Number:

46-1371-82

Clone Number:

17B5

41BB is an important co-stimulatory molecule and is analyzed on T cells in the tumor to determine potency of the anti-tumor response.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor lymphocytes

Primary Incubation

1:400

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

Can easily identify 41BB+ T cells in the tumor.

DOI or PMID #

N/A

Additional Notes

Titrate antibody based on tissue samples

Related Categories

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Summary

The Good

Robustly identifies CD137+ T cells in the tumor.

The Bad

None

The Bottom Line

Awesome and reliable antibody that fits nicely in aurora panels if studying T cells in the tumor.

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