Great Flow Antibody to Detect CTLA4 Expression on T Cells

January 21, 2021

BlueskyReddit
Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher eBioscience

Product Name:

CD152 (CTLA-4) Monoclonal Antibody-PE-eFluor 610

Catalog Number:

61-1522-82

Clone Number:

UC10-4B9

CTLA4 is a critical negative regulator on T cells. This antibody identifies exhausted T cells in the tumor along with other co-inhibitory molecules.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor lymphocytes

Primary Incubation

1:400

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

Can easily identify truly exhausted CTLA4+ T cells in the tumor.

DOI or PMID #

NA

Additional Notes

This antibody is part of the intracellular staining protocol step.

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Summary

The Good

Robustly identifies exhausted T cells in the tumor.

The Bad

None

The Bottom Line

Awesome reliable antibody to detect CTLA4+ T cells in the tumor.

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