Great Antibody to Identify CD8 T Cells Via Flow Cytometry

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Anti-Mouse CD8a BV480

Catalog Number:

566096

Clone Number:

53-6.7

Info: Supplier Page 

Browse Similar Products: CD8a Flow Cytometry Antibodies

As an immunologist studying T cells, this antibody is reliable to identify CD8 T cells from various tissues in our daily experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Tumor lymphocytes

Primary Incubation

1:400

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

We are readily able to detect CD8+ T cells in our tumor samples using flow cytometry.

DOI or PMID #

NA

Additional Notes

Titrate antibody based on tissue samples

Related Categories

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Summary

The Good

Robustly identifies CD8 T cells in the tumor.

The Bad

None.

The Bottom Line

I highly recommend this antibody to anyone studying T cells in tissues as it provides a strong, consistent labeling of CD8+ T cells for flow cytometry experiments.

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