Lambda 10-3 is an Automatic Multi-filter Wheel System for Multi-Wavelength Imaging

TIRF microscopy was performed using an Olympus IX81 motorized inverted microscope with 60×/1.45 oilimmersion objective and Lambda 10–3 filter wheel containing 480/40, 540/30, and 575lp emission filters. CFP, YFP, and mCherry were excited by 445-, 514-, and 561-nm lasers, respectively, via cell^TIRF Control 1.3 software. Cell images were collected every 5 s using an ORCA-Flash4.0 camera and MetaMorph software.