Biolegend
Brilliant Violet 510™ anti-mouse/human CD44 Antibody
103043
IM7
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B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to titrate anti-CD44.
Flow Cytometry
wild-type mouse spleen
surface staining with, CD44 BV510 (1/100), CD19, etc (20 min, 4° in the dark)
clone 2.4G2 (Fc block)
N/A
Flow cytometry, violet laser
I could stain CD44+ cells in the spleen using this antibody at 1/100.
The antibody works well.
None
It can be used