Pierce ECL Plus as a Substrate for Western Blot

Medical University of Graz
Molecular Biology and Biochemistry
Research Scientist

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Company:

Thermo Scientific

Product Name:

Pierce ECL Plus Western blotting substrate

Catalog Number:

32134

We measured the level of pp65 in LPS treated BV2 cells, by western blot. We used Pierce ECL Plus for detecting the bands.This substrate gave good bands of pp65.

Experimental Design and Results Summary

Application

Western blot

Starting Material

BV2 cells

Tips

Protect from light while putting the reagent in the blot

Results Summary

We obtained good bands of phosphorylated form of p65 with this substrate

DOI or PMID #

N/A

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Summary

The Good

Protein which are in less amount can also be detected well.

The Bad

Sometimes the bands of highly expressed protein get oversaturated

The Bottom Line

A good western blot detection substrate, especially when the protein of interest is at low concentration

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