Good CD3 Monoclonal Antibody from Miltenyi

November 06, 2020

Lehrstuhl Neurologie
University Regensburg
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD3 Antibody, anti-human, FITC

Catalog Number:

130-113-128

Clone Number:

N/A

We are studying the CNS involvement of mycosis fungoides (cuteneous T cell lymphoma). Here, we analyzed the presence of CD3 positive cells in mycosis fungoides by using the MACSima multi staining system.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (lymphoma)

Primary Incubation

1:20 for 1 h at room temperature

Blocking Agent

3% BSA in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence microscopy

Results Summary

Antibody gives a nice staining of CD3 cells

DOI or PMID #

N/A

Additional Notes

N/A`

Related Categories

Image Gallery

Summary

The Good

Reliable & reproducible staining

The Bad

Minor background signals

The Bottom Line

Works as expected

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