MyBioSource.com
p53 Antibody
MBS395752
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To test the time course effect of particular growth factor on p53 expression in mouse, the primary cell lines were growth-arrested for 12 hrs. in medium devoid of FBS. The synchronized cells treated without or with growth factor, prepared protein extracts, and an equal amount of proteins were resolved in 10%SDS-PAGE. The resolved proteins were then western transferred in a nitrocellulose membrane and probed with p53 specific antibody. Although, MyBioSource.com supply a polyclonal antibody, the selection of Ab was based on the merits indicated in antibody flyer.
Western Blot
Mouse primary cell whole cell lysate
Dilution (1:1000) in 5% BSA prepared in 1x TBST, overnight incubation at 4 degree Celsius
skimmed milk (5%) prepared in 1x TBST
Goat anti-sheep IgG HRP, dilution (1:4000) in 5% skimmed milk prepared in 1x TBST, 2hr at RT
None
ECL detection in X-Ray film
p53 from MyBioSource.com was a polyclonal antibody and it has detected a very faint band of p53 protein. Interestingly, a non-highly specific band with high Intensity was observed at higher molecular weight.
none
Low specificity of Ab for p53
Antibody highly cross-reacted with other nonspecific epitopes
Bad antibody for p53 detection