Thermo Fisher Scientific
Pierce™ BCA Protein Assay Kit
23225
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We measured the protein content of the cell lysate using this kit to normalise our results. 10ul of the sample was mixed with 200ul of the reagent (50:1)and after brief shaking, was incubated at 37 degree C for 30 min.The absorbance was measured at 562 nm.
Protein estimation
Standard-Bovine Serum Albumin (BSA), Sample- Cell lysate
N/A
The standard curve of BSA was used to calculate the protein content in the cell lysate.
Easy, fast
It is an easy method for protein estimation in cell lysates.