Biolegend
APC anti-mouse CD272 (BTLA) Antibody
134809
8F4
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Browse Similar Products: CD272 Flow Cytometry Antibodies
It is well known that B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to titrate anti-CD272 (BTLA) that is expressed in B cells and not in T cells.
Flow Cytometry
Wild-type mouse spleen
surface staining with anti-CD272 (1/100) (20 min, 4° in the dark)
clone 2.4G2 (Fc block)
N/A
Flow cytometry, red laser
B cells are stained at 1/100 dilution
It works
Not very bright
It can be used.