Easy F-actin Detection

University of Pittsburgh
Immunology
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Scientific

Product Name:

Alexa Fluor™ 488 Phalloidin

Catalog Number:

A12379

Using Thermo Scientific's Alexa Fluor 488 Phallodin makes F-actin detection a breeze. Useful for many types of imaging to mark the cell walls and have a clean border for cytoplasm.

Experimental Design and Results Summary

Application

Immunofluorescence imaging

Starting Material

4% PFA fixed D.10 cells

Tips

Reconstituted in MeOH for a 40x stock per manufacturers instructions, 20-60m incubation at room temperature

Results Summary

Easily marks the border of my cells for good compartment analysis.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to use, great signal and very reliable staining.

The Bad

None.

The Bottom Line

Easily mark cell boundaries with this F-actin stain, I've been using for close to a decade and will continue to use.

Join the discussion