Cell Signaling Technology (CST)
Rabbit Anti-Histone H3-phospho (Ser10)
#9701
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Good antibody to detect the phosphorylation at Ser10 of histone H3
Western Blot
Hela cells, cell lysate
1:1000 milk 5% in PBS 0,05% tween
Milk 5% in PBS 0,05% tween
rabbit 1:3000 milk 5% in PBS 0,05% tween 45 minutes
None
Ecl
The phosphorylation of the H3 indicates mitosis and meiosis activity, therefore the antibody was used to detect the effects exerted on cell cycle upon transfection with the gene of our interest.
Good signal
Highly recommended