Isotype Control for Flow Cytometry

Molecular Biology and Biochemistry
Medical University of Graz
Research Scientist

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Quality of Results

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Company:

Thermo Fisher Scientific

Product Name:

Rabbit IgG Isotype Control, FITC

Catalog Number:

11-4614-80

Clone Number:

N/A

BV2 microglia cells were treated with LPS and stained with isotype control and compared with unstained and GLUT1-FITC stained cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

BV2 mouse microglia cells

Primary Incubation

Isotype control was incubated with 1:250 dilution

Blocking Agent

Ultravision protein block

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Guava easyCyte flowcytometer

Results Summary

Isotype control didn't result in much change as compared to unstained control. Whereas, GLUT1-FITC increased the precentage of GLUT1 positive cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good quality

The Bad

Didn't find any

The Bottom Line

The isotype control confirmed the binding specificity of the antibody.

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