Alomone labs
Anti-GLUT1 (extracellular)-FITC Antibody
AGT-041-F
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BV2 microglial cells were treated with LPS, and then detected for extracellular GLUT1 by this antibody by flowcytometry.
Flow Cytometry
mouse BV2 microglia cell line
Cells were collected with PBS. After blocking, it was it was incubated with the antibody at 1:500 dilution
Ultravision Protein block
It is directly conjugated, therefore doesn't require secondary incubation
NA
It was detected using Guava easyCyte flowcytometer
LPS increased the percentage of GLUT1 positive cells compared to control cells.
1:500 worked better than 1:1000
It is directly conjugated with FITC
Didn't find any
It is a good antibody to detect extracellular GLUT1. Since it is directly conjugated, it reduces the time of processing the sample