Rabbit Anti-Pax6 Antibody for Immunofluorescence

October 09, 2020

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Hiss diagnostic

Product Name:

Rabbit Anti-Pax6 antibody

Catalog Number:

PRB-278P

Clone Number:

N/A

Mouse embryonic Spinal cord sections E12.5 labeled by this Anti-Pax6 antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse spinal cord: E12.5

Primary Incubation

1:50, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal Microscopy.

Results Summary

Cheap and decent antibody to detect Pax6+ cells. Reliable staining, however it can sometimes be weak and lower dilutions needs to be used.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Price and good signal.

The Bad

None.

The Bottom Line

For the price and quality of this antibody, I would recommend its use.

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