LRRC26 for Western Blot

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Alomone labs

Product Name:

Anti-LRRC26 Antibody

Catalog Number:

APC-070

Clone Number:

N/A

Leucine-rich repeat containing protein 26 (otherwise called as BK channel auxiliary gamma subunit or LRRC26) is a 37 kDa single spanning transmembrane protein. It has five major leucine rich repeats in its extracellular domain which are all required for modulating BK channel activity. The single transmembrane segment of LRRC26 is required for co-assembly with the BKα subunit complex. Here we checked the time course effect of LRRC26 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

Observed time dependent increase of LRRC26 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This antibody is very good for Western Blot experiments.

The Bad

None

The Bottom Line

It works great for western blot. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion